control lentiviral vectors Search Results


lentiviral vectors expressing nonsense control (lenti-nc)  (Shanghai GenePharma)
90
Shanghai GenePharma lentiviral vectors expressing nonsense control (lenti-nc)
Lentiviral Vectors Expressing Nonsense Control (Lenti Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/lentiviral+vectors+expressing+nonsense+control++lenti+nc+/pmc06492646-86-5-20
Average 90 stars, based on 1 article reviews
lentiviral vectors expressing nonsense control (lenti-nc) - by Bioz Stars, 2026-09
90/100 stars
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lentiviral vectors carrying gfp and either shrna targeting gpr171 mrna or a non-targeting control shrna  (Shanghai GenePharma)
90
Shanghai GenePharma lentiviral vectors carrying gfp and either shrna targeting gpr171 mrna or a non-targeting control shrna
GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control <t>shRNA‐treated</t> and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.
Lentiviral Vectors Carrying Gfp And Either Shrna Targeting Gpr171 Mrna Or A Non Targeting Control Shrna, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/lentiviral+vectors+carrying+gfp+and+either+shrna+targeting+gpr171+mrna+or+a+non+targeting+control+shrna/pmc12050395-122-12-17
Average 90 stars, based on 1 article reviews
lentiviral vectors carrying gfp and either shrna targeting gpr171 mrna or a non-targeting control shrna - by Bioz Stars, 2026-09
90/100 stars
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lentiviral transfer vectors encoding bfp-2a-stop (control)  (Cellectis sa)
90
Cellectis sa lentiviral transfer vectors encoding bfp-2a-stop (control)
GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control <t>shRNA‐treated</t> and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.
Lentiviral Transfer Vectors Encoding Bfp 2a Stop (Control), supplied by Cellectis sa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/lentiviral+transfer+vectors+encoding+bfp+2a+stop++control+/pmc06554542-299-0-23
Average 90 stars, based on 1 article reviews
lentiviral transfer vectors encoding bfp-2a-stop (control) - by Bioz Stars, 2026-09
90/100 stars
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lentiviral vectors to silence twist2 together with the negative control (shnc)  (Shanghai GenePharma)
90
Shanghai GenePharma lentiviral vectors to silence twist2 together with the negative control (shnc)
GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control <t>shRNA‐treated</t> and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.
Lentiviral Vectors To Silence Twist2 Together With The Negative Control (Shnc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/lentiviral+vectors+to+silence+twist2+together+with+the+negative+control++shnc+/pm25088197-186-4-13
Average 90 stars, based on 1 article reviews
lentiviral vectors to silence twist2 together with the negative control (shnc) - by Bioz Stars, 2026-09
90/100 stars
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lentiviral vectors containing human ulbp-1 or non-target control shrna  (Shanghai GenePharma)
90
Shanghai GenePharma lentiviral vectors containing human ulbp-1 or non-target control shrna
GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control <t>shRNA‐treated</t> and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.
Lentiviral Vectors Containing Human Ulbp 1 Or Non Target Control Shrna, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/lentiviral+vectors+containing+human+ulbp+1+or+non+target+control+shrna/pmc11301968-72-0-13
Average 90 stars, based on 1 article reviews
lentiviral vectors containing human ulbp-1 or non-target control shrna - by Bioz Stars, 2026-09
90/100 stars
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lentiviral vectors expressing small interfering rnas (sirnas) directed against negative control (lenti-nc)  (Shanghai GenePharma)
90
Shanghai GenePharma lentiviral vectors expressing small interfering rnas (sirnas) directed against negative control (lenti-nc)
GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control <t>shRNA‐treated</t> and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.
Lentiviral Vectors Expressing Small Interfering Rnas (Sirnas) Directed Against Negative Control (Lenti Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/lentiviral+vectors+expressing+small+interfering+rnas++sirnas++directed+against+negative+control++lenti+nc+/pmc05364886-63-5-19
Average 90 stars, based on 1 article reviews
lentiviral vectors expressing small interfering rnas (sirnas) directed against negative control (lenti-nc) - by Bioz Stars, 2026-09
90/100 stars
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lentiviral vectors expressing negative control shrna (len-si-nc)  (BioVector Inc)
90
BioVector Inc lentiviral vectors expressing negative control shrna (len-si-nc)
GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control <t>shRNA‐treated</t> and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.
Lentiviral Vectors Expressing Negative Control Shrna (Len Si Nc), supplied by BioVector Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/lentiviral+vectors+expressing+negative+control+shrna++len+si+nc+/pm39696562-32-7-18
Average 90 stars, based on 1 article reviews
lentiviral vectors expressing negative control shrna (len-si-nc) - by Bioz Stars, 2026-09
90/100 stars
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lentiviral vectors expressing hs2st1 or the control (nt)  (SignaGen)
90
SignaGen lentiviral vectors expressing hs2st1 or the control (nt)
GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control <t>shRNA‐treated</t> and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.
Lentiviral Vectors Expressing Hs2st1 Or The Control (Nt), supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/lentiviral+vectors+expressing+hs2st1+or+the+control++nt+/pmc11277508-189-7-29
Average 90 stars, based on 1 article reviews
lentiviral vectors expressing hs2st1 or the control (nt) - by Bioz Stars, 2026-09
90/100 stars
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lentiviral vectors of hsa-mir-negative control  (Shanghai GenePharma)
90
Shanghai GenePharma lentiviral vectors of hsa-mir-negative control
GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control <t>shRNA‐treated</t> and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.
Lentiviral Vectors Of Hsa Mir Negative Control, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/lentiviral+vectors+of+hsa+mir+negative+control/pm30483755-51-28-35
Average 90 stars, based on 1 article reviews
lentiviral vectors of hsa-mir-negative control - by Bioz Stars, 2026-09
90/100 stars
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lentiviral vectors carrying a mirna mimic/inhibitor or nonsense controls (nc2)  (Shanghai GenePharma)
90
Shanghai GenePharma lentiviral vectors carrying a mirna mimic/inhibitor or nonsense controls (nc2)
GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control <t>shRNA‐treated</t> and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.
Lentiviral Vectors Carrying A Mirna Mimic/Inhibitor Or Nonsense Controls (Nc2), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/lentiviral+vectors+carrying+a+mirna+mimic+inhibitor+or+nonsense+controls++nc2+/pmc05958633-95-28-34
Average 90 stars, based on 1 article reviews
lentiviral vectors carrying a mirna mimic/inhibitor or nonsense controls (nc2) - by Bioz Stars, 2026-09
90/100 stars
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its control lentiviral vector  (Shanghai GenePharma)
90
Shanghai GenePharma its control lentiviral vector
GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control <t>shRNA‐treated</t> and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.
Its Control Lentiviral Vector, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/its+control+lentiviral+vector/pmc09360216-148-7-25
Average 90 stars, based on 1 article reviews
its control lentiviral vector - by Bioz Stars, 2026-09
90/100 stars
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lentiviral vectors lv1-egfp (control) or lv1-egfp-mir-7  (GenScript corporation)
90
GenScript corporation lentiviral vectors lv1-egfp (control) or lv1-egfp-mir-7
GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control <t>shRNA‐treated</t> and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.
Lentiviral Vectors Lv1 Egfp (Control) Or Lv1 Egfp Mir 7, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+lentiviral+vectors/lentiviral+vectors+lv1+egfp++control++or+lv1+egfp+mir+7/pm38446664-251-10-34
Average 90 stars, based on 1 article reviews
lentiviral vectors lv1-egfp (control) or lv1-egfp-mir-7 - by Bioz Stars, 2026-09
90/100 stars
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GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Helicobacter

Article Title: HIF ‐1α‐Induced GPR171 Expression Mediates CCL2 Secretion by Mast Cells to Promote Gastric Inflammation During Helicobacter pylori Infection

doi: 10.1111/hel.70042

Figure Lengend Snippet: GPR171 partially mediates CCL2 production via the ERK1/2 pathway. (A–C) GPR171 expression was verified in GPR171‐knockdown RBL‐2H3 cells by RT‐qPCR and western blotting. (D) Percentage of β‐hexosaminidase released into the supernatants from control shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells in response to H. pylori infection. (E) The mRNA levels of the 6 cytokines in control shRNA‐treated and GPR171 shRNA‐treated cells stimulated with H. pylori . (F) CCL2 levels in the supernatants of shRNA‐treated and GPR171 shRNA‐treated RBL‐2H3 cells co‐cultured with H. pylori . (G) GO enrichment of DEGs based on RNA‐seq data. (H) Immunoblot analysis and quantification of ERK1/2 phosphorylation in RBL‐2H3 cells that were or were not treated with H. pylori . p‐ERK expression was normalized to total ERK. (I) RT‐qPCR analysis of CCL2 mRNA expression in mast cells co‐cultured with or without H. pylori . (J) CCL2 levels in supernatants were quantified by ELISA. Cells were pretreated with 5 μM U0126 as indicated (H–J). (K) Immunoblot analysis and quantification of p‐ERK in control shRNA‐treated and GPR171 shRNA‐treated cells following H. pylori stimulation. (L, M) Western blot analysis of ERK1/2 phosphorylation expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . (N, O) RT‐qPCR and ELISA analysis of CCL2 expression in RBL‐2H3 cells treated with different concentrations of MS21570 (0, 5, 10, and 20 μM) and co‐cultured with or without H. pylori . MS21570: A blocker of GPR171. Statistical significance was determined by Student's unpaired t ‐test (A, C, D, E, F, K) and one‐way ANOVA (H, I, J, M, N, O), * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Lentiviral vectors carrying GFP and either shRNA targeting GPR171 mRNA or a non‐targeting control shRNA were customized (GenePharma, China).

Techniques: Expressing, Knockdown, Quantitative RT-PCR, Western Blot, Control, shRNA, Infection, Cell Culture, RNA Sequencing, Phospho-proteomics, Enzyme-linked Immunosorbent Assay